Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Optimization of extraction of total flavonoids from Drynaria rhizome, and its effect on osteoclast differentiation

Bo Yuan, Fang Yu, WenJi Song, ZhengNan Zhao, HaiDong Liang

Hands and Feet Microsurgery, The Second Hospital of Dalian Medical University, Dalian, 116023, Peopleâ€™s Republic of China;

For correspondence:- HaiDong Liang Email: lianghddlf@sina.com

Accepted: 25 March 2019 Published: 30 April 2019

Citation: Yuan B, Yu F, Song W, Zhao Z, Liang H. Optimization of extraction of total flavonoids from Drynaria rhizome, and its effect on osteoclast differentiation. Trop J Pharm Res 2019; 18(4):829-835 doi: 10.4314/tjpr.v18i4.21

© 2019 The authors.
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Abstract

Purpose: To optimize the extraction parameters for total flavonoids of Drynaria rhizome, and determine their effect on osteoclast differentiation and bone resorption in vitro.

Methods: A 5-level 3-factor central composite design was applied to the optimization of extract yield of total flavonoids from Drynaria rhizome. Response Surface Methodology (RSM) design was used to optimize total flavonoids extraction from Drynaria rhizome. The independent factors included extraction temperature (A), extraction time (B) and liquid: solid ratio (C). Moreover, NFATc1, DC-STAMP, cathepsin K and MMP-9 mRNA expressions were determined.

Results: Values obtained were fitted into a second-order polynomial equation with multiple regression analysis using a statistical method. Analysis of variance results indicate that the independent variables influenced total flavonoid extraction from Drynaria rhizome. The optimal conditions for extraction yield were extraction temperature of 75 ^oC, extraction time of 100 min, and liquid: solid ratio of 107:1. The yield of 5.38 ± 0.62 % was consistent with these optimized conditions, which was an indication of the accuracy of the model. Experiments revealed that total flavonoids from Drynaria rhizome regulated the expression levels of NFATc1, DC-STAMP, cathepsin K and MMP-9 mRNA

Conclusion: This study has successfully optimized the extraction yield of total flavonoids from Drynaria rhizome. The total flavonoids inhibit osteoclast differentiation and bone resorption. Thus, they may be beneficial in the treatment of bone diseases.

Keywords: Flavonoids, Drynaria rhizome, RSM, Osteoclast differentiation, Bone resorption